Diffusion tensor imaging (DTI) is a non-invasive promising technique for longitudinal study in the biomedical field. Physician and pharmacists apply this biomarker to evaluate the efficiency of drugs and to improve the analysis of preclinical experiments such as spinal repair. However, interleaved DTI sequence carried out at ultra high field are impaired by motion and off-resonance effects. In this case, this biomarker can not be validated for biomedical applications. The purpose is to suggest a new strategy for a robust acquisition and to confirm this potential biomarker.